To See Or Not To See...Can Non-Cooled sCMOS Cameras Do the Job?
In the years since their first appearance in 2010, scientific cameras based on scientific CMOS image sensors have had a large impact on numerous new technologies and methods in microscopy. Practically all camera based methods employing super resolution and nanoscopy have made use of the ideal combination, of low readout noise, high quantum efficiency and high frame rates associated with a very convenient pixel pitch of 6.5 µm, which fit perfectly to many microscope applications.
First Clinical Applications on the Horizon for FLIM
From microscopy of ocular diseases to imaging in neurosurgery to tomography in patients with skin cancer and brain tumors, flurorescence lifetime imaging microscopy is the versatile partner that can be teamed with a host of the other imaging techniques to produce striking results.
Piezo Drives in the Real-Time Autofocus System
High-Speed microscopy for quality control.
Fluorescence Lifetime Imaging Made Easy
Fluorescence Lifetime Imaging Microscopy (FLIM), which measures the spatial distribution of the fluorescence decay, can be achieved either in the time or in the frequency domain. Each approach exhibits advantages and disadvantages, depending on the type of light detector and acquistiton method employed.
sCMOS Cameras Examine Movements of Pathogens
PCO has been developing high-performance scientific cameras for over 25 years. Gerhard Holst explains how its sCMOS image sensors are aiding scientific advancement
Scientific CMOS camera technology: A breeding ground for new microscopy techniques
In 2009 a new type of complimentary metal-oxide semiconductor (CMOS) imagesensorwas developed by a a consortium of three companies: Fairchild Imaging (now a division of BAE Systems), Andor Technology and PCO . At the time the partners decided to call the device a scientific CMOS (sCMOS for short) image sensor to make clear from the very beginning that the performance data of this new type of sensor would be very different from the usual standard CMOS image sensor. The combination of high sensitivity, low readout noise, high frame rate, high resolution and high dynarnic range did not exist before, not even within the commonly used cooled CCD and electron multiplying CCD cameras, which were widely available for microscopy.
Determination of fluorescence lifetime made easy. Lifetime or decay rate relates to the phenomenon of fluorescence. This process, photoluminescence, is widely utilized across many areas, for tagging cells and cell fragments in order to observe metabolic processes, for differentiating between reaction products or for the characterization of any parameters that induce a change in the fluorescence.
Lifetime reloaded (DE)
Fluoreszenzabklingzeiten mit weniger Aufwand bestimmen. Lifetime, Lebensdauer oder Abklingzeit sind Begriffe, die mit dem Phänomen der Fluoreszenz, der spontanen Emission von Licht kurz nach der Anregung des Materials, zu tun haben. Dieser Vorgang, die sogenannte Photolumineszenz, wird heutzutage in vielen Bereichen genutzt, um Zellen oder Zellbestandteile zu markieren, um Stoffwechselprozesse zu visualisieren, um Reaktionsprodukte zu differenzieren oder um mithilfe der Änderung der Lumineszenz andere Parameter wie zum Beispiel den Sauerstoffpartialdruck zu messen.
The Innovation of Images
An interview with the head of the research department of PCO.
25 years at the top
After a quarter century in the business, PCO is broadening its appeal beyond the research community.
Fluorescent Imaging of Optogenetic Pacemaker Cells for the Heart
Optogenetics uses light to stimulate geneitcally modified cells. Researchers at Stony Brook University plan to use these cells as optical cardiac pacemakers. A dedicated highspeed camera is being employed to monitor the work of the cells, providing relevant insight into the propagation patterns of the activation signal.
The German Aerospace Center (DLR) has developed a camera that can take 3D pictures of Earth. And it can do IT from a jet airplane traveling at very high speed.
3D at 2000 Hz at 300 km/h
High-Speed Video Analysis for Motorsport
New digital cameras make image capture swifter and easier at Arnold Air Force Base ballistic range
Stereo Vision for Airbags
A new method allows for the creation of a 3D model of the airbag expansion process without the need for locally identifiable pattern codes
Scientific CMOS Camera
From Micro to Macro...
Noise in Imaging: the Good, the Bad, and the Right
For imaging applications, it's important to know what kind of noise you're dealing with.
Darkness on the Edge of Your Images
Large imaging sensors bring special challenges.
New options for better systems
10 questions and answers about the chances and consequences of the new EMVA 1288 standard for camera system characterization.
CMOS cameras rise to speed challenge
Cameras based on CMOS image sensors are providing a new cost-effective way to
perform high-speed imaging. Gerhard Holst describes the latest technology on offer.
Development and Application of a MHz Frame Rate
The ability to capture the unsteady dynamics of high speed compressible flows requires the use of ultra fast imaging diagnostics.
Fast Camera Images of Flux Ropes During Plasma Relaxation
IEEE Transactions on Plasma Science - Images of visible light emission from flux ropes during plasma relaxation.
View to the future
CCD and CMOS sensors today and tomorrow
Analysis of visible light images
Review of Scientific Instruments - Analysis of visible light images from a fast-gated intensified charge coupled device camera during flux rope interaction and magnetic reconnection
Ophtalmoscope enters digital age
Opto & Laser Europe - article about the application of a color pixelfly camera system in a new digital opthalmoscope.
Electron Multiplication CCDs Offer a Flexible Solution
The technology advances signal amplification but has its own limitations.
Allows rapid data transfer - new technology bypasses PC storage.
12 bit dynamics
Applications in machine vision. The once huge difference in speed or frame rate between digital 8 and 12-bit camera systems has now vanished due to continuous improvements in image sensor manufacturing and microprocessors. What is left is simply a certain price difference and the question of whether 12- bit camera systems can be used for machine vision and if so, the criteria defining these applications.
Imaging on a Gland Scale
BioPhotonics - Some results of a study of the neuroendocrine system done with various microscope techniques and using a sensicam qe camera system.
PLIF technique for fuel visualization in engines and its role in retrofit technology
Colorado State University (CSU) Engines and Energy Conversion Laboratory (EECL) - Planar Laser Induced Fluorescence (PLIF) for combustion analysis using a dicam pro for measurements.
Fluorescence Imaging advances fuel-injector design
Laser Focus World - Planar Laser-Induced Fluorescence (PLIF) is used to improve the fule injection of an engine. The images were recorded with a dicam pro.
Red enables cell membrane study
BioPhotonics - Cell membrane investigations by fluorescence microscopy using a sensicam qe for the recording of images.
Camera captures spheromak plasmas
Photonics Spectra - The fusion of plasmas in an experimental spheromak reactor were imaged using a dicam pro.
A novel method for instantaneous, quantitative measurement of molecular mixing in gaseous flows
Experiments in Fluids 2002, No.33 - Laser induced fluorescence measurements using fluorescence and phosphorescence recorded with a dicam pro.
Study of Magnetic Helicity Injection via Plasma Imaging using a High-Speed Digital Camera
California Institute of Technology - The evolution of a plasma generated by a novel panar coaxial gun is photographed using a hsfc pro, which captures eight time resolved images per discharge.
A Laboratory Plasma Experiment for Studying Magnetic Dynamics of Accretion Discs and Jets
Monthly Notices of the Royal Astronomical Society - An hsfc pro reveals images of the formation and helical instability of a collimated plasma, similar to MHD models of disc jets and also plasma detachment associated with spheromak formation.
Transparent oxygen optodes in environmental applications at fine scale as measured by luminescence l
Proc. SPIE Vol. 4576 - The article shows various applications with a modular luminescence lifetime imaging system (MOLLI) and 2D and 3D mapping of oxygen with corresponding optodes. The system is based on a sensicam sensimod.
Luminescence Lifetime Imaging with Transparent Oxygen Optodes
Sensors & Actuators B - Imaging of the 2D distribution od oxygen using planar oxygen optodes and a modular luminescence lifetime imaging system (MOLLI) based on a sensicam seensimod camera system.
Laser Focus World - Specialized techniques based on high-speed digital imaging allow analysis of instantaneous impacts.
Getting better flow-structure information
NASA Tech Briefs - Digital particle image velocimetry using a sensicam double shutter.
Smear in CCD sensors
Smear in CCD sensors.