Multiprobe Fluorescence Imaging
Fluorescence images (false color coded) showing Indain muntjac deer epidermis fibroblast cells stained with multiple probes.
Fluorescence Lifetime Imaging (FLIM) with a focus on frequency domain FLIM with pco.flim
This video tutorial explains how fluorescence lifetime imaging (FLIM) can be done and what the benefit of frequency domain FLIM done with a pco.flim camera system is.
Some stunning slow motion sequences of young kingfishers trying to catch fish, recorded by Rainer Bergomaz from Blue Paw Artists with a pco.dimax HD+ highspeed camera at 1920 x 1440 pixel resolution @ 1060 frames/s.
Slow motion sequences of fishing kingfishers with partially underwater view, taken with a pco.dimax HD+ by Rainer Bergomaz from Blue Paw Artists.
Frog jumps to the water
A frog jumps into the water, recorded at 2000 frames per second with full HD resolution.
Birds in slow motion
A couple of sequences showing bird flights in slow motion were recorded with pco.dimax high speed cameras in Full HD. The frame rate varied between 500 frames/s - 2000 frames/s.
Luminescence lifetime imaging camera
This video shows the principle of photoluminescence in the time domain (pulsed excitation) and in the frequency domain (continuous, modulated excitation) using the pco.flim – a specialized camera for luminescence lifetime measurements in the frequency domain. The corresponding waveforms of light, shaped and delayed by a reflective target and a photoluminescent sample, are visualized. In the time domain a short light pulse excites a photoluminescent sample, which emits a decaying light pulse. In the frequency domain, the same sample introduces a phase shift to the luminescent response of the periodically modulated excitation light. This particular phase shift can be detected by the pco.flim, which provides a dedicated modulatable image sensor.
pco.edge structured illumination
Photonik Campus - VDI on the Hannover Exhibition
During the Hannover exhibition in april 2014 we have been present at the Photonik Campus booth of the VDI, which tried to attract boys and girls for photonics. A variety of situations was generated to record highspeed clips, which were directly processed after the moment and given to the visitors as a slow motion clip on a memory stick as a thank you for their contribution. Here a selection is shown...
A great tit approaches a birdhouse, lands, leaves again and returns. During this return the flight looks a bit like a ballistic "flying" with wings folded. The sequence was recorded at 1500 frames/s at Full HD with a pco.dimax highspeed camera. It is displayed in 25 frames/s while the scenes in the birdhouse run four times faster. In total four seconds have been recorded. Since it was recorded from inside some reflections in the window are unfortunately visible.
Ladybugs Take Off...
Various sequences of ladybugs, which prepare and take off for flying away. The sequences were recorded by cameraman Rainer Bergomaz from Blue Paw Artists with a pco.dimax HD at 3000 frames/s and 1296 x 720 pixel resolution. Within the sequences the before and after parts are displayed at 250 frames/s and when the ladybug starts to unfold its wings the display speed is reduced to 25 frames/s. And a sequence with a water drop falling onto a flower is included.
scientific CMOS pco.edge - high dynamic and sensitivity (sCMOS)
A low light sequence out of the lab window was taken in the dawn at 100 frames/s and 2560 x 2160 Pixel with our scientific CMOS (sCMOS) color camera pco.edge. The sequence is displayed three times at different levels of 16bit to 8bit scaling to show the details, which the pco.edge is able to detect in available light scenes.
Protein Networks in Water Drops in an Oil Phase
A sequence of fluorescence labeled protein networks in water drops in an oil phase is shown, which moved fast. One pixel corresponds to 0.1625 μm in reality (false color rendering). It has been recorded with a pco.edge 5.5 sCMOS camera. Courtesy of Prof. Dr. Sarah Köster, Institute for X-Ray Physics, Georg-August-University, Göttingen, Germany.
Sea-Eagle - in slow motion
A Sea-Eagle from a falconry starts, flies and lands in slow motion. The sequences were filmed by Norbert Porta from Science Docu (sciencedocu.de) with a pco.dimax at 1000 frames/s in 2016 x 2016 pixel resolution. A Full HD extract is shown at various display speeds from 25 - 100 images/s.
Strokkur Eruption at Midnight - in Slow Motion
A photo and video artist has recorded this eruption of the gush spring Strokkur at midnight and the nice daylight pan shot of the waterfall Hraunfossar. Both recorded in Iceland with a pco.dimax HD highspeed camera in Full HD resolution (1920 x 1080 pixel). Both sequences are displayed at varying speeds.