Die Fluoreszenzbilder (Falschfarben kodiert) zeigen die Epidermis Fibroblast Zellen eines indischen Muntjac Hirsches, die mit mehreren Markern angefärbt wurden.
Fluorescence Lifetime Imaging (FLIM) with a focus on frequency domain FLIM with pco.flim
This video tutorial explains how fluorescence lifetime imaging (FLIM) can be done and what the benefit of frequency domain FLIM done with a pco.flim camera system is.
Eisvögel beim Fischen II
Rainer Bergomaz von Blue Paw Artists sind eindrucksvolle Aufnahmen von fischenden Eisvögeln gelungen. Er hat sie mit einer pco.dimax HD+ Highspeed-Kamera beobachtet und sie dabei mit 1920 x 1440 Pixeln Auflösung bei 1060 Bildern/s aufgenommen.
Eisvögel beim fischen
Zeitlupensequenzen von fischenden Eisvögeln mit Unterwasseransichten. Diese wurden mit einer pco.dimax HD+ von Rainer Bergomaz von Blue Paw Artists aufgenommen.
Ein Frosch springt ins Wasser
Zeitlupenaufnahme mit 2000 Bildern/s bei Full HD Auflösung, in der ein Frosch in ein Aquarium springt.
Vögel in Zeitlupe
Eine Folge von Bildsequenzen, die verschiedene Vögel im Flug in Zeitlupe in Full-HD zeigen und mit pco.dimax Hochgeschwindiskeitskameras aufgenommen wurden. Die Bildrate variert hier von 500 Bildern/s bis zu 2000 Bildern/s.
Luminescence lifetime imaging camera
This video shows the principle of photoluminescence in the time domain (pulsed excitation) and in the frequency domain (continuous, modulated excitation) using the pco.flim – a specialized camera for luminescence lifetime measurements in the frequency domain. The corresponding waveforms of light, shaped and delayed by a reflective target and a photoluminescent sample, are visualized. In the time domain a short light pulse excites a photoluminescent sample, which emits a decaying light pulse. In the frequency domain, the same sample introduces a phase shift to the luminescent response of the periodically modulated excitation light. This particular phase shift can be detected by the pco.flim, which provides a dedicated modulatable image sensor.
pco.edge structured illumination
Photonik Campus - VDI on the Hannover Exhibition
During the Hannover exhibition in april 2014 we have been present at the Photonik Campus booth of the VDI, which tried to attract boys and girls for photonics. A variety of situations was generated to record highspeed clips, which were directly processed after the moment and given to the visitors as a slow motion clip on a memory stick as a thank you for their contribution. Here a selection is shown...
A great tit approaches a birdhouse, lands, leaves again and returns. During this return the flight looks a bit like a ballistic "flying" with wings folded. The sequence was recorded at 1500 frames/s at Full HD with a pco.dimax highspeed camera. It is displayed in 25 frames/s while the scenes in the birdhouse run four times faster. In total four seconds have been recorded. Since it was recorded from inside some reflections in the window are unfortunately visible.
Ladybugs Take Off...
Various sequences of ladybugs, which prepare and take off for flying away. The sequences were recorded by cameraman Rainer Bergomaz from Blue Paw Artists with a pco.dimax HD at 3000 frames/s and 1296 x 720 pixel resolution. Within the sequences the before and after parts are displayed at 250 frames/s and when the ladybug starts to unfold its wings the display speed is reduced to 25 frames/s. And a sequence with a water drop falling onto a flower is included.
scientific CMOS pco.edge - high dynamic and sensitivity (sCMOS)
A low light sequence out of the lab window was taken in the dawn at 100 frames/s and 2560 x 2160 Pixel with our scientific CMOS (sCMOS) color camera pco.edge. The sequence is displayed three times at different levels of 16bit to 8bit scaling to show the details, which the pco.edge is able to detect in available light scenes.
Protein Networks in Water Drops in an Oil Phase
A sequence of fluorescence labeled protein networks in water drops in an oil phase is shown, which moved fast. One pixel corresponds to 0.1625 μm in reality (false color rendering). It has been recorded with a pco.edge 5.5 sCMOS camera. Courtesy of Prof. Dr. Sarah Köster, Institute for X-Ray Physics, Georg-August-University, Göttingen, Germany.
Sea-Eagle - in slow motion
A Sea-Eagle from a falconry starts, flies and lands in slow motion. The sequences were filmed by Norbert Porta from Science Docu (sciencedocu.de) with a pco.dimax at 1000 frames/s in 2016 x 2016 pixel resolution. A Full HD extract is shown at various display speeds from 25 - 100 images/s.
Strokkur Eruption at Midnight - in Slow Motion
A photo and video artist has recorded this eruption of the gush spring Strokkur at midnight and the nice daylight pan shot of the waterfall Hraunfossar. Both recorded in Iceland with a pco.dimax HD highspeed camera in Full HD resolution (1920 x 1080 pixel). Both sequences are displayed at varying speeds.