Microarrays are versatile tools for high throughput screening. Nevertheless they are severely limited. Therefore the idea arose why not to copy microarrays? Why not make DNA, RNA and protein microarrays as high quality copies of a high quality original? It worked fine for text books and images. So why not apply it for DNA? Why not build a biomolecule copying machine? A biomolecule xeroxer?
DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important.
Oblique plane microscopy (OPM) is a form of light sheet microscopy that uses a single high numerical aperture microscope objective for both fluorescence excitation and collection.
The application shows that the mode and dynamics of trypanosome locomotion are a trait of life within a crowded environment. Using high-speed fluorescence microscopy and ordered micro-pillar arrays we show that the parasites mode of motility is adapted to the density of cells in blood.
Widefield frequency-domain fluorescence lifetime imaging microscopy (FDFLIM) is a fast and accurate method to measure the fluorescence lifetime of entire images.
Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM).
Luminescence Lifetime Imaging of Chemical Sensors - A Comparison between Time-Domain and Frequency-Domain Based Camera Systems
Achieving a maximal safe extent of resection during brain tumor surgery is the goal for improved patient prognosis. Fluorescence-guided neurosurgery using 5-aminolevulinic acid (5-ALA) induced Protoporphyrin IX has thereby become a valuable tool enabling a high frequency of complete resections and a prolonged progression-free survival in glioblastoma patients.
Novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic receptor groups inducing fluorescence quenching via photoinduced electron transfer mechanism.
With the help of the pco.dimax S4, the characteristics behind bone fractures are investigated. The goal is to understand the material as well as tissue level strain limits and their relation to failure sites.
With the Confocal.nl RCM2, super-resolution imaging over a large FOV is possible. Here we can observe resolved mouse synaptonemal complexes over an incredible 220x220 micron FOV. Using the pco.edge 26 camera and an Olympus Life Science 40x 1.4 NA objective.